Disease model | Modeling approach | Animal strains | Measurements |
Sepsis model | Intraperitoneal injection of LPS | SD/Wistar rat | Renal pathological analysis; Serum cytokine analysis; blood bacterial culture |
Cecal ligation and puncture |
Severe acute pancreatitis (SAP)model | Induced by retrograde infusion of 5% sodium taurocholate solution (1 mg/kg) into the biliary-pancreatic duct | SD/Wistar rat | Pancreatic histopathological observation; Analysis of serum AMY and LIP; Detection of inflammatory factors |
Deafness model | Gene knockout, heterozygous breeding | C57BL/6 mouse | Tone and intensity testing |
Depression model | Forced swimming, heat stress, etc | SD/Wistar rat | Animal behavior judgment |
Aplastic anemia(AA) model | administered with 2ml/Kg of benzene for 15 dose | CD1 mouse | Blood tests; Bone marrow tests |
Injection of 5-FU with busulfan | SD/Wistar rat |
Received irradiation+ tail vein injection of Lymph cells | BALB/c mouse |
Cervical spondylotic radiculopathy (CSR) model | Surgery+formaldehyde filter paper was placed under 6/7 nerve roots | SD/Wistar rat | Detection of pathological changes around nerve roots |
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Case analysis Sepsis rat model
Sepsis, a severe systemic inflammatory response as a result of infection, is associated with high morbidity and mortality rates, and remains one of leading cause of death in intensive care units worldwide due to its association with the dysfunction of multiple organs. Rats were subjected to cecal ligation and puncture (CLP) surgery, corresponding to mortality of human patients suffering from abdominal sepsis.
Experimental animal information |
Name of animal model | Sepsis rat model |
Species and strains of animal | SD/ Wistar rat |
Gender of animal | ♂ |
Age of animal | adult |
Weight of animal | 180~250g |
Caracteristics
Compared to the sham control, Serum TNF-α, IL-6 and IL-1β were elevated in CLP rats, the infiltration of inflammatory cell in the Liver, lung, kidney ; The myocardial cells were loosely arranged in heart tissue; Small intestinal mucosa were characterized: edema, hemorrhage with inflammatory cell infiltration.
Partial surgical process display
