Disease model | Modeling approach | Animal strains | Measurements |
Guinea pig model of asthma | Intraperitoneal injection of 10%OVA sensitization +OVA continuous atomization excitation | Hartleyguinea pig | Breathing pattern analysis; Total cells and eosinophils counts were performed in BALF; Histological assessment of lung |
Chronic bronchitis rat model | Passive smoking | SD/Wistar rat | Histological assessment of lung、Analysis of inflammatory cell infiltration in bronchial wall and base; Analysis of alveolar septal lesions |
Chronic obstructive pulmonary disease rat model (COPD-rat) | Repeatedly instilled with LPS intratracheally on the first day and on the 14th day; On days 2–13 days and 15–28 days, the rats were placed in a self-produced fumigating box for smoking | SD/Wistar rat | the forced expiratory volume in 0.3 s/forced vital capacity (FEV0.3/FVC%) was lower and inspiratory resistance (RI) was higher in COPD model; Histological assessment of lung |
Pneumonia rat model | Tail vein injection of 10mg/kg LPS | Wistar rat | Lung injury, macroscopic observation of large area bleeding exudation; Histological assessment of lung; Detection of inflammatory factors |
Pulmonary emphysema mouse model | intratracheal instillation of pancreatic porcine elastase | C57BL/6 mice | fractional area of alveolar collapse, and hyperinflated alveoli increased; Elastic fiber content decreased; Collagen fiber content increased; Lung inflammation |
Pulmonary fibrosis rat model | Bleomycin was intratrachally aspirated | SD/Wistar rat | Histological assessment of lung; Inflammatory cells count in BALF; Hydroxyproline detection |
guinea pig model of allergic rhinitis(AR) | Intranasally ovalbumin sensitization | Hartley guinea pig | Sneezing and nose rubbing; Nasal blockade, leukocyte infiltration, and lung inflation pressure |
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Case analysis Pulmonary fibrosis rat model
Idiopathic pulmonary fibrosis (IPF) is a chronic, diffuse, progressive disease of the pulmonary interstitium with agnogenic etiology, and it lacks effective therapy in the clinic now. Now, experimental research of IPF commonly induces the model by intratracheal injection of bleomycin (BLM) in rodents, It has been observed in the experimental model induced by BLM that the histopathologic changes of lung tissue were mainly inflammation of alveoli in early stage and then gradual progression of fibrosis deposition.
Experimental animal information |
Name of animal model | Pulmonary fibrosis rat model |
Species and strains of animal | SD/ Wistar rat |
Gender of animal | ♂ |
Age of animal | adult |
Weight of animal | 180~220g |
Caracteristics
Control group revealed normal lung tissue structure and slight collagen deposition in the alveolar septa. Treatment with BLM revealed severe collagen deposition, obliteration of interalveolar spaces, and disordered lung structure. Alveolar space on day 28 was almost occluded by fibrosis and normal alveoli were observed scarcely. Hydroxyproline in BLM group were significantly higher when compared to that of control group. Total cell count increased in the BALF of BLM treated rats compared with that in the BALF of control rats, The total number of macrophages and neutrophils significantly increased in BLM treated rats.
Partial result display
Masson’s trichrome (MT) stain（×200）
Determination of hydroxyproline content
