卡卡湾厅

High throughput sequencing (High-throughput sequencing), also known as "next generation" sequencing, which can sequence hundreds of thousands to millions of DNA molecules in parallel.

Real-time fluorescence quantitative PCR (PCR) is a method of adding fluorescent groups to PCR reaction system and using fluorescence signal accumulation to real-time monitor the total amount of products after each (PCR) cycle of polymerase

The small RNA is the regulating RNA with molecule of 1928 nt, which mainly includes micro RNA (micro RNA,miRNA) and small interference RNA (short interfering RNA,siRNA).

SiRNA is a small RNA with molecule of ~ 21-25 nucleotides, which is produced by Dicera specific enzymeto double stranded RNA in the RNAaseⅢ family.

SNP, is called single nucleotide polymorphism, which mainly refers to the DNA sequence polymorphism caused by single nucleotide variation at the genomic level.

DNA methylation is refer to change 5’cytosineof CpG dinucleotide to methylcytosine through induced by DNA methyltransferase (DNMTs) , and is one of the earliest approaches.

Site-directed mutagenesis is the introduction of desired changes (usually changes in the favorable direction) into the desired DNA fragments (either genome or plasmid) by means of polymerase chain reaction (PCR),

Vector construction is to transport the target DNA molecule to the recipient cells, so we must find a carrier that can enter the cell and load the foreign DNA fragment into the cell, and still copy it in the same way.